Composite

Part:BBa_J100204:Design

Designed by: Monica Prudencio   Group: Campbell M Lab   (2014-12-05)


actClone Red


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 1739
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 1739
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 1739
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 1739
    Illegal AgeI site found at 1612
    Illegal AgeI site found at 1724
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 928
    Illegal BsaI.rc site found at 817
    Illegal SapI site found at 708


Design Notes

The stop codons of the RBS and start codons of the proteins overlap to increase effective translation. This is cloned into pSB1A8 with modified Bba prefix so that there is only an EcoRI site upstream and a normal Bba suffix.


Source

The two main indicator proteins are GFP (BBa_I746916) and RFP (BBa_E1010).

References